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Ribonomics of the chloroplast basal expression apparatus: ribosomes and RNA Polymerases

In this project, we are tagging the chloroplast encoded RNA polymerase and the chloroplast ribosome to allow immunological enrichment of RNAs bound to these molecular machines. RNAs associated with these two machineries serves as a direct read-out of nascent and translated mRNAs, respectively. Applying this technique to different conditions and developmental stages will help to identify regulatory points in plastid transcription and translation.