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Equipment |
| Olympus Fluoview FV-1000 |
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| Inverted Laser Scanning microscope | ||
| Single photon point scanning Olympus
FV1000 confocal microscope with three fluorescence and one transmitted
light detectors PicoQuant Fluorescence Lifetime Microscopy (FLIM) upgrade with two SPAD detectors
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| special features | confocal imaging: multicolor parallel and sequential
imaging, line scan, point scan, Z stacks, time-series, advanced
time-series, Kalman averaging, summarizing, scan zoom and rotation highly efficient scan mode with fast switching between imaging and activation for bleaching, photoactivation, FRAP Time resolved fluorescence: Fluorescence Lifetime Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS) |
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| microscope | based on Olympus IX-81 inverted fully motorized fluorescence microscope, transmitted light with DIC or phase contrast (100x) |
| objectives | 10x/0.4 UPlanApo, DIC 20x/0.7 UPlanApo, DIC 40x/1.35 UPlanSApo, DIC 60x/1.35 Oil UPlanSApo, DIC 100x/1.35 Oil UPlanSApo, Phase contrast 60x/1.2 Water UPlanSApo |
| illumination | Halogen HBO 405nm diode laser 458, 488, 515 ArgonLaser 561 nm diode laser |
| detection | point scanner, one pinhole, 3 confocal PMTs: two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection one filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting |
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reflectors for widefield observation & TIRFM |
Filter Cube 2.
U-MWU2: BP330-385, BA420, DM400 Filter Cube 3. U-MCFPET: BP426-446, BA460-500, DM455 Filter Cube 4. U-MNB2: BP470-490, BA520IF, DM500 Filter Cube 5. U-MWG2: BP510-550, BA590, DM570 Filter Cube 6. DIC |
| dichroic filters for LSM | 80/20 405/488/559/635 405-458/515/559/635 458/515 405/458/515 |
PicoQuant Fluorescence Lifetime Microscopy (FLIM) upgrade |
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| illumination picosecond-pulsed lasers |
405nm diode laser 440nm diode laser 470nm diode laser 635nm diode laser |
| detection | two higly sensitive (SPAD) detectors resolvable time resolution: < 0.5 ns |
| incubation | incubation chamber for temperature (RT-37°C), CO2 and
humidity temperature controlled holder for 35mm dishes and slights (waterbath controlled) |
| more |
Olympus information on FV1000 with free download of FV-1000 viewer software, can be used to view, arrange and convert Olympus image files |