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Equipment |
| Olympus Fluoview FV-1000MPE |
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| Inverted Laser Scanning microscope | ||
| Point scanning Olympus
FV1000 confocal microscope with single & multiphoton excitation, three
confocal fluorescence detectors, two non-descanned detectors and one transmitted
light detector Total Internal Reflection Microscopy (TIRFM) module from Olympus with Orca ER camera
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| special features | confocal imaging: multicolor parallel and sequential
imaging, line scan, point scan, Z stacks, time-series, advanced
time-series, Kalman averaging, summarizing, scan zoom and rotation highly efficient scan mode with fast switching between imaging and activation for bleaching, photoactivation, FRAP TIRM: selective visualization of the specimen immediately adjacent to the bottom cover slip (up to about 100nm) Multiphoton excitation for imaging in tissues ond organs with high penetration depth |
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| microscope | based on Olympus IX-81 inverted fully motorized fluorescence microscope, transmitted light with DIC or phase contrast (100x) |
| objectives | 10x/0.4 UPlanApo, DIC 20x/0.7 UPlanApo, DIC 40x/1.35 UPlanSApo, DIC 60x/1.2 Water UPlanSApo, DIC 60x/1.45 Oil UPlanSApo - for TIRFM |
| illumination | Halogen HBO 405nm diode laser 440nm diode laser (458), 488, 515 ArgonLaser 561 nm diode laser 635 nm diode laser |
| detection | point scanner, one pinhole, 3 confocal PMTs: two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection one filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting 2 non-descanned detectors for multiphoton imaging |
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reflectors for widefield observation & TIRFM |
Filter Cube 2. tripple-band filter DAPI/FITC/TRIC Filter Cube 3. double-band cfp/yfp Filter Cube 6. DIC |
| dichroic filters for LSM | 80/20 405-458/515/559/635 405/488/559/635 458/515 405/458/515 |
Total Internal Reflection Microscopy (TIRFM) module from Olympus |
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| reflectors | Filter Cube 4. IVEVA (TIRFM cfp) Filter Cube 5. IBEVA (TIRFM gfp) |
| detection | Hamamatsu Orca ER cooled CCD camera seperate imaging computer with Olympus Cell-R software |
| incubation (planned) | incubation for temperature (RT-37°C) and CO2 temperature controlled holder for 35mm dishes and slights (waterbath controlled) available |
| more |
Olympus information on FV1000MPE with free download of FV-1000 viewer software, can be used to view, arrange and convert Olympus image files |