fast switching between imaging and activation for bleaching, photoactivation, FRAP
TIRM: selective visualization of the specimen immediately adjacent to the bottom cover slip (up to about 100nm)
| Visitron VisiScope |
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| Inverted Scanning Disc Laser Confocal Microscope | ||
| Fully motorized Olympus IX83 microscope with
Yokogawa CSU-W1 Confocal Spinning Disc unit and excitation by diode
lasers, two highly sensitive cameras, VisiTirf
Total Internal Reflection Microscopy (TIRFM) module, VisiFrap module for
photo activation / bleaching of freely defined regions in programmed
sequences or on the fly.
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| special features | confocal and epifluorescence multicolor
imaging, fast Z-stacks, time-series, advanced
time-series, fast switching between imaging and activation for bleaching, photoactivation, FRAP TIRM: selective visualization of the specimen immediately adjacent to the bottom cover slip (up to about 100nm) |
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| microscope | based on Olympus IX-83 inverted fully motorized
fluorescence microscope, transmitted light with DIC Ludl EP Bioprecision2 motorized x-y-stage Ludl EP NanoPrecision fast Piezo Z-stage insert |
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| objectives | 20x/0.75 LWD PlanApo, DIC 30x/1.05 UPlanSApo, Silicon, DIC 40x/0.95 UPlanSApo, DIC 60x/1.2 UPlanSApo, Water, DIC 60x/1.45 UPlanSApo, Oil (TIRFM) 150x/1.47 UPlanSApo, Oil (TIRFM) |
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| illumination | Halogen HBO 405 nm diode laser 445 nm diode laser 488 nm diode Laser 561 nm diode laser 640 nm diode laser |
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reflectors for widefield observation & imaging |
Filter Dapi (ET 350/50, T 400LP, ET 460/50) Filter CFP (ET 436/20, T 455LP, ET 480/40) Filter GFP (ET 470/40, T 495LPXR, ET 525/50) Filter TRIC (ET 560/40, T 585LP, ET 630/75) Filter CY5 (ET 640/30, T 660LP, ET 690/50) Filter CY7 (ET 710/75, T 760LPXR, ET 810/90) |
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| Yokogawa CSU-W1 Confocal Spinning Disc unit | ||||
| dichroic filters for CSU | 405/488/559/635 nm 445/515/640 nm |
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| emission filters camera 1 (Evolve) |
Dapi ET460/50 nm |
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| emission filters camera 2 (pco.edge) |
Dapi
ET460/50 GFP ET525/50 CFP ET470/24 mCherry ET600/50 CY5 ET700/75 |
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| detection | 2 cameras: (1) Andor iXon Ultra 888 back-illuminated EM-CCD, 1024x1024 pixels, 13x13 μm, 26 fps (2) pco.edge 4.2 sCMOS, 2048x2048 pixels, 6.5x6.5 μm, max. 100 fps |
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| Total Internal Reflection Microscopy (TIRFM)
dichroic filters |
405/488/561/640 nm 442/514 |
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| incubation | incubation for temperature (RT-37°C) and CO2 |
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| more | Olympus IX83 | Yokogawa CSU-X1 | VisiTIRF | VisiFrap |