In a great collaboration with the group of Nicholas Taylor, we present the structure of nature’s smallest rotary motor - the stator units that power bidirectional rotation of the bacterial flagellum. Published in Cell!
See below for a movie of the mechanistic model for how the stator units can power bidirectional rotation of the flagellar motor.
We show that posttranslational methylation of the flagellar filament contributes to efficient adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces. Published in Nature Communications!
Very happy and honored to receive funding for our project BacNanoMachine from the European Research Council!
We are happy to get support from the DFG to purchase a new superresolution microscope!
Superresolution microscopy is needed in order to investigate cellular structures and dynamic cellular processes below the Abbe diffraction limit. The new STED microscope will serve the need for superresolution light microscopy on a nanometer-scale (nanoscopy) of several research groups at the Institute for Biology.
Check out some example images below, where we have detected the flagellar filament and hook structure using STED microscopy and compared the images to the normal confocal microscopy mode. In contrast to the diffraction-limited confocal mode, STED microscopy allowed us to precisely measure on a nanometer-scale the size of the flagellar hook structure, which – according to electron microscopy experiments – grew to a length of ∼55 nm and substantially increased the spatial resolution of the immunostained flagellar filaments
We demonstrate that bacteria control the optimal length of the flagellar hook structure to nanometer-scale in order to maximize stability of the flagellar bundle. Published in PLoS Biology in collaboration with Yann Dufour and Wilson Poon!
We show that the degenerate EAL domain protein RflP responds to cell envelope stress and subsequently downregulates flagella biosynthesis. Published in mBio!
In collaboration with Kelly T. Hughes and David F. Blair at the University of Utah, we show that the secretion pore of the flagellar type-III protein export apparatus is formed by the protein FliP. Published in Molecular Microbiology!
Florian's paper on the role of FliO as a flagellum-specific chaperone has been published in PLoS Biology! Congrats!
The microbiota composition determines the susceptibility towards Salmonella-induced gastroenteritis. A collaborative work together with Till Strowig at the Helmholtz Centre for Infection Research. Published in Cell Host & Microbe!
Thibaud's paper on the mechanism of filament growth has been published in eLife! Congrats Thibaud!