• Home |
• Contact |
• Sitemap |
• Imprint |
• Privacy policy |
• Accessibility |
• Deutsch |

• Faculties
  • Breeding
    • General
    • Projects
  • Microbiology and diseases of the bee
    • General
    • Projects
  • Bee Products
    • General
    • Projects
• Contacts
  • Staff
  • Address
  • Imprint
• Beebreed.eu

Utilisation of gene expression differences in hygienic and non-hygienic working bees towards Varroa-parasitised brood for the selection of generally disease-resistant honey bees

Experts unanimously consider the early detection and removal of infested brood as the most effective control strategy against brood diseases. There are validated results that a different sensitivity to the fragrance bouquet of diseased brood plays a central role in hygienic behaviour, regardless of the type of pathogen.The basis for the proposed gene expression analyses are long-term infrared video recordings of individually marked worker bees with a very different hygienic behaviour towards Varroa and chalkbrood-infested brood. In addition to that, opto-physiological methods (Ca-imaging) applied to animals with highly differentiated behaviour allow for an investigation of the steps important for the detection of odour profiles and which organs (antennae, antennal lobe, mushroom bodies) are particularly relevant to individual behavioral differences. The differences in behaviour can be described directly as differences in gene expression. For this, the transcription differences of approximately 12,000 genes are represented in parallel using the microarray method. The bioinformatic analysis of the differences identifies relevant reaction pathways that are associated with the differences in behaviour. Corresponding candidate genes of the reaction pathways are verified by means of Q-PCR and are in part functionally tested.

Publications:

• Bienefeld, K. (2009) HyBee - Entwicklung Molekulargenetischer Methoden für die Selektion krankheits resistenter Honigbienen basierend auf Genexpressionsunterschieden von hygienischen und nicht-hygienischen Arbeitsbienen. Proc. 2. FUGATO - Statusseminar, p 20.
• Rudolf, H.; Gempe,T.Leboulle, L. Beye,M., Bienefeld, K.; Reinsch, K. (2009) Verzerrungen in Microarray-Analysen gepoolter RNA-Proben Proc. 2. FUGATO - Statusseminar, p 45.
• Schöning, C.; Bienefeld, K. (2009) Hygieneverhalten von Honigbienen gegenüber Varroa parasitierter Brut. Proc. 2. FUGATO - Statusseminar, p 46.
• Gempe, T., Leboulle, G., Bienefeld, K., Beye, M. (2009) Identification of genes differentially expressed between hygienic and non-hygienic honeybees. Proc. 2. FUGATO - Statusseminar, p 47.
• Chakroborty, N.K., Eisenhardt, D.; Bienefeld, K.; Schöning, C.; Menzel, R. (2009) Understanding the contribution of the olfactory chemoreception to elicit the hygienic behaviour of the European honeybee Apis mellifera against the Varroa destructor. Proc. 2. FUGATO - Statusseminar, p 48.
• Rudolf, H., Gempe, T., Beye, M., Bienefeld, K., Reinsch, N.:(2009) Einfluss variabler Poolgrößen  in der Analyse der Genexpression von Zweifarben-Arrays. Proc. Jahrestagung der DGfZ und GfT am 16./17. September 2009 in Gießen, D19
• Bienefeld, K., Schöning, C.  Menzel, R. Leboulle, G., Chakraborty, N., Beye, M. Gempe, T. Reinsch, N. Rudolf, H. (2011) HyBee - Entwicklung Molekulargenetischer Methoden für die Selektion krankheitsresistenter Honigbienen basierend auf Genexpressionsunterschieden von hygienischen und nicht-hygienischen Arbeitsbienen Proc. 3. FUGATO-Statusseminar 2011, p 12.
• Schöning, C.;  Bienefeld, K. (2011) Verhaltensexperimente zur Phänotypisierung von hygienischen Arbeitsbienen bezüglich Milben (Varroa destructor) und dem Kalkbrut-Pilz (Ascosphaera apis) Proc. 3. FUGATO-Statusseminar 2011, p 59.
• Gempe, T., Leboulle, G., Bienefeld, K., Beye, M. (2011) Analyse von Genexpressionsunterschieden zwischen hygienischen und nicht-hygienischen Honigbienen. Proc. 3. FUGATO-Statusseminar 2011, p 60.
• Chakroborty, N.; Georgieva, P.; Lehman, ,K.; Bienefeld , K.; Schöning, C. Menzel R.  (2011) Behavioral and physiological studies to discern the possible differences in olfactory perception between hygienic and non-hygienic honeybee (Apis mellifera) colonies Proc. 3. FUGATO-Statusseminar 2011, p 61.
• Schöning, C.; Gisder, S.; Geiselhardt, S.; Kretschmann, I.; Bienefeld, K.; Hilker, M.; Genersch, E. (2012) Evidence for damage-oriented hygienic behaviour towards Varroa destructor-parasitised brood in the western honey bee Apis mellifera L. The Journal of Experimental Biology 215, 264-271

back