9 daughter colonies of the 4a were tested:
DE-4-252-1-2005 DE-4-252-2-2005 DE-4-252-3-2005 DE-4-252-4-2005 DE-4-252-5-2005 DE-4-252-6-2005 DE-4-252-10-2005 DE-4-252-11-2005 DE-4-252-12-2005
13 tested queens were mated at the mating station:
DE-4-252-11-2007 DE-4-252-12-2007 DE-4-252-13-2007 DE-4-252-14-2007 DE-4-252-15-2007 DE-4-252-16-2007 DE-4-252-17-2007 DE-4-252-18-2007 DE-4-252-19-2007 DE-4-252-20-2007 DE-4-252-21-2007 DE-4-273-56-2007 DE-4-273-62-2007
6 daughter colonies of queens mated at the mating station were tested:
DE-4-252-11-2008 DE-4-252-12-2008 DE-4-252-13-2008 DE-4-252-14-2008 DE-4-252-15-2008 DE-4-252-16-2008