11 daughter colonies of the 4a were tested:
DE-16-24-558-1983 DE-16-24-559-1983 DE-16-24-560-1983 DE-16-24-561-1983 DE-16-24-562-1983 DE-16-24-563-1983 DE-16-24-564-1983 DE-16-24-565-1983 DE-16-24-566-1983 DE-16-24-567-1983 DE-16-24-569-1983
23 tested queens were mated at the mating station:
DE-16-24-601-1984 DE-16-24-603-1984 DE-16-24-605-1984 DE-16-24-606-1984 DE-16-24-607-1984 DE-16-24-608-1984 DE-16-24-610-1984 DE-16-24-611-1984 DE-16-24-613-1984 DE-16-24-614-1984 DE-16-24-615-1984 DE-16-24-616-1984 DE-16-24-619-1984 DE-16-24-620-1984 DE-16-24-621-1984 DE-16-91-32784-1984 DE-16-91-32884-1984 DE-16-91-32984-1984 DE-16-91-33084-1984 DE-16-91-33184-1984 DE-16-91-33284-1984 DE-16-91-33384-1984 DE-16-91-33484-1984
15 daughter colonies of queens mated at the mating station were tested:
DE-16-24-690-1986 DE-16-24-691-1986 DE-16-24-692-1986 DE-16-24-693-1986 DE-16-24-694-1986 DE-16-24-695-1986 DE-16-24-696-1986 DE-16-24-724-1987 DE-16-24-725-1987 DE-16-24-726-1987 DE-16-24-728-1987 DE-16-24-729-1987 DE-16-24-730-1987 DE-16-24-731-1987 DE-16-24-732-1987