10 daughter colonies of the 4a were tested:
DE-16-303-7-2001 DE-16-303-9-2001 DE-16-303-10-2001 DE-16-303-16-2001 DE-16-303-17-2001 DE-16-303-18-2001 DE-16-303-24-2001 DE-16-303-26-2001 DE-16-303-28-2001 DE-16-303-30-2001
10 tested queens were mated at the mating station:
DE-16-303-7-2001 DE-16-303-9-2001 DE-16-303-10-2001 DE-16-303-16-2001 DE-16-303-17-2001 DE-16-303-18-2001 DE-16-303-24-2001 DE-16-303-26-2001 DE-16-303-28-2001 DE-16-303-30-2001
15 daughter colonies of queens mated at the mating station were tested:
DE-16-303-10-2003 DE-16-303-14-2003 DE-16-303-15-2003 DE-16-303-16-2003 DE-16-303-18-2003 DE-16-303-22-2003 DE-16-303-24-2003 DE-16-303-27-2003 DE-16-303-31-2003 DE-16-303-33-2003 DE-16-303-34-2003 DE-16-303-36-2003 DE-16-303-37-2003 DE-16-303-39-2003 DE-16-303-40-2003