10 daughter colonies of the 4a were tested:
DE-2-147-13-2002 DE-2-147-15-2002 DE-2-147-16-2002 DE-2-147-18-2002 DE-2-147-19-2002 DE-2-147-20-2002 DE-2-147-22-2002 DE-2-147-44-2002 DE-2-147-48-2002 DE-2-147-49-2002
18 tested queens were mated at the mating station:
DE-2-147-77-2004 DE-2-147-78-2004 DE-2-147-80-2004 DE-2-147-81-2004 DE-2-147-85-2004 DE-2-147-86-2004 DE-2-222-1-2004 DE-2-222-17-2004 DE-2-222-22-2004 DE-2-222-25-2004 DE-2-222-27-2004 DE-2-222-34-2004 DE-2-222-35-2004 DE-2-222-37-2004 DE-2-222-42-2004 DE-2-222-44-2004 DE-2-222-53-2004 DE-2-222-54-2004
5 daughter colonies of queens mated at the mating station were tested:
DE-2-222-99-2007 DE-2-222-106-2007 DE-2-222-110-2007 DE-2-222-113-2007 DE-2-222-123-2007