12 daughter colonies of the 4a were tested:
DE-2-217-1-2009 DE-2-217-2-2009 DE-2-217-3-2009 DE-2-217-4-2009 DE-2-217-5-2009 DE-2-217-7-2009 DE-2-217-8-2009 DE-2-217-9-2009 DE-2-217-10-2009 DE-2-217-11-2009 DE-2-217-14-2009 DE-2-217-16-2009
10 tested queens were mated at the mating station:
DE-2-217-1-2010 DE-2-217-18-2010 DE-2-217-19-2010 DE-2-217-20-2010 DE-2-217-21-2010 DE-2-217-22-2010 DE-2-217-23-2010 DE-2-217-24-2010 DE-2-217-25-2010 DE-2-217-118-2010
19 daughter colonies of queens mated at the mating station were tested:
DE-2-217-1-2014 DE-2-217-4-2014 DE-2-217-5-2014 DE-2-217-6-2014 DE-2-217-7-2014 DE-2-217-9-2014 DE-2-217-12-2014 DE-2-217-13-2014 DE-2-217-14-2014 DE-2-217-55-2013 DE-2-217-59-2013 DE-2-217-61-2013 DE-2-217-62-2013 DE-2-217-63-2013 DE-2-217-71-2013 DE-2-217-72-2013 DE-2-217-73-2013 DE-2-217-74-2013 DE-2-217-75-2013