21 daughter colonies of the 4a were tested:
IT-20-108-131-2019 IT-20-108-133-2019 IT-20-108-134-2019 IT-20-108-135-2019 IT-20-108-136-2019 IT-20-108-139-2019 IT-20-108-1016-2020 IT-20-108-3011-2018 IT-20-108-3028-2018 IT-20-108-4017-2019 IT-20-108-4032-2019 IT-20-108-4040-2019 IT-20-108-4050-2019 IT-20-108-6009-2020 IT-20-108-6012-2020 IT-20-108-6014-2020 IT-20-108-6025-2020 IT-20-108-6028-2020 IT-20-108-6042-2020 IT-20-108-6049-2020 IT-20-108-6050-2020
170 tested queens were mated at the mating station:
DE-4-1-4-2021 DE-4-1-5-2021 DE-4-1-11-2021 DE-4-1-14-2021 DE-4-1-15-2021 DE-4-1-16-2021 DE-4-1-17-2021 DE-4-1-18-2021 IT-20-68-104-2021 IT-20-68-105-2021 IT-20-68-107-2021 IT-20-68-108-2021 IT-20-68-109-2021 IT-20-68-112-2021 IT-20-68-113-2021 IT-20-68-1011-2021 IT-20-68-1013-2021 IT-20-68-1016-2021 IT-20-68-1020-2021 IT-20-68-1024-2021 IT-20-68-1025-2021 IT-20-68-1026-2021 IT-20-68-1048-2021 IT-20-68-1049-2021 IT-20-68-1050-2021 IT-20-68-1056-2021 IT-20-68-1066-2021 IT-20-68-1071-2021 IT-20-68-1072-2021 IT-20-68-1073-2021 IT-20-68-1074-2021 IT-20-68-1075-2021 IT-20-70-114-2021 IT-20-70-115-2021 IT-20-70-116-2021 IT-20-70-117-2021 IT-20-70-119-2021 IT-20-70-120-2021 IT-20-70-122-2021 IT-20-70-123-2021 IT-20-87-34-2021 IT-20-87-36-2021 IT-20-87-37-2021 IT-20-87-38-2021 IT-20-87-40-2021 IT-20-87-41-2021 IT-20-87-42-2021 IT-20-87-43-2021 IT-20-101-55-2021 IT-20-101-56-2021 IT-20-101-57-2021 IT-20-101-58-2021 IT-20-101-59-2021 IT-20-101-60-2021 IT-20-101-61-2021 IT-20-101-62-2021 IT-20-101-63-2021 IT-20-101-64-2021 IT-20-101-65-2021 IT-20-106-66-2021 IT-20-106-69-2021 IT-20-106-71-2021 IT-20-106-72-2021 IT-20-106-73-2021 IT-20-106-74-2021 IT-20-106-75-2021 IT-20-106-76-2021 IT-20-106-3013-2021 IT-20-106-3056-2021 IT-20-108-23-2021 IT-20-108-24-2021 IT-20-108-25-2021 IT-20-108-26-2021 IT-20-108-27-2021 IT-20-108-28-2021 IT-20-108-29-2021 IT-20-108-30-2021 IT-20-108-31-2021 IT-20-108-32-2021 IT-20-108-7032-2021 IT-20-108-7056-2021 IT-20-110-134-2021 IT-20-110-135-2021 IT-20-110-137-2021 IT-20-110-140-2021 IT-20-110-141-2021 IT-20-110-143-2021 IT-20-118-44-2021 IT-20-118-46-2021 IT-20-118-49-2021 IT-20-118-50-2021 IT-20-118-51-2021 IT-20-118-52-2021 IT-20-118-53-2021 IT-20-118-54-2021 IT-20-118-1025-2021 IT-20-118-4025-2021 IT-20-145-15-2021 IT-20-145-27-2021 IT-20-145-46-2021 IT-20-145-54-2021 IT-20-145-58-2021 IT-20-145-77-2021 IT-20-145-93-2021 IT-20-145-94-2021 IT-20-145-99-2021 IT-20-145-164-2021 IT-20-145-166-2021 IT-20-145-168-2021 IT-20-145-169-2021 IT-20-145-170-2021 IT-20-145-172-2021 IT-20-145-173-2021 IT-20-145-174-2021 IT-20-158-77-2021 IT-20-158-78-2021 IT-20-158-79-2021 IT-20-158-80-2021 IT-20-158-81-2021 IT-20-159-93-2021 IT-20-159-97-2021 IT-20-159-98-2021 IT-20-159-100-2021 IT-20-159-102-2021 IT-20-163-156-2021 IT-20-163-157-2021 IT-20-163-158-2021 IT-20-163-160-2021 IT-20-163-162-2021 IT-20-163-163-2021 IT-20-163-1111-2021 IT-20-163-1113-2021 IT-20-236-144-2021 IT-20-236-145-2021 IT-20-236-146-2021 IT-20-236-147-2021 IT-20-236-149-2021 IT-20-236-150-2021 IT-20-236-151-2021 IT-20-236-152-2021 IT-20-236-153-2021 IT-20-236-154-2021 IT-20-236-2002-2021 IT-20-236-2004-2021 IT-20-236-2005-2021 IT-20-236-2006-2021 IT-20-236-2007-2021 IT-20-236-2008-2021 IT-20-236-2011-2021 IT-20-236-2012-2021 IT-20-236-2070-2021 IT-20-245-83-2021 IT-20-245-84-2021 IT-20-245-85-2021 IT-20-245-87-2021 IT-20-245-88-2021 IT-20-245-90-2021 IT-20-245-91-2021 IT-20-245-92-2021 IT-20-282-22-2021 IT-20-282-29-2021 IT-20-282-125-2021 IT-20-282-126-2021 IT-20-282-127-2021 IT-20-282-128-2021 IT-20-282-129-2021 IT-20-282-130-2021 IT-20-282-131-2021 IT-20-282-132-2021 IT-20-282-133-2021
13 daughter colonies of queens mated at the mating station were tested:
IT-20-145-3-2022 IT-20-145-4-2022 IT-20-145-35-2022 IT-20-145-56-2022 IT-20-145-75-2022 IT-20-145-78-2022 IT-20-145-88-2022 IT-20-145-139-2022 IT-20-145-140-2022 IT-20-145-142-2022 IT-20-145-145-2022 IT-20-145-147-2022 IT-20-145-569-2022