21 daughter colonies of the 4a were tested:
DE-2-176-53-2009 DE-2-176-55-2009 DE-2-176-57-2009 DE-2-176-59-2009 DE-2-176-61-2009 DE-2-176-64-2009 DE-2-176-67-2009 DE-2-176-68-2009 DE-2-176-70-2009 DE-2-176-71-2009 DE-2-176-72-2009 DE-2-176-73-2009 DE-2-176-75-2009 DE-2-176-426-2009 DE-2-176-576-2009 DE-2-176-577-2009 DE-2-176-922-2009 DE-2-176-1036-2009 DE-2-176-1039-2009 DE-2-176-1040-2009 DE-2-176-1043-2009
29 tested queens were mated at the mating station:
IT-20-72-93-2010 IT-20-72-97-2010 IT-20-72-100-2010 IT-20-84-76-2010 IT-20-84-77-2010 IT-20-84-78-2010 IT-20-84-101-2010 IT-20-84-105-2010 IT-20-108-2-2010 IT-20-108-3-2010 IT-20-108-6-2010 IT-20-108-7-2010 IT-20-108-8-2010 IT-20-108-108-2010 IT-20-118-67-2010 IT-20-118-68-2010 IT-20-118-70-2010 IT-20-118-72-2010 IT-20-118-73-2010 IT-20-118-75-2010 IT-20-161-80-2010 IT-20-161-83-2010 IT-20-161-87-2010 IT-20-161-90-2010 IT-20-163-59-2010 IT-20-163-60-2010 IT-20-163-61-2010 IT-20-163-63-2010 IT-20-163-65-2010
49 daughter colonies of queens mated at the mating station were tested:
IT-20-101-65-2013 IT-20-101-66-2013 IT-20-101-67-2013 IT-20-101-68-2013 IT-20-101-69-2013 IT-20-101-70-2013 IT-20-108-1-2012 IT-20-108-2-2012 IT-20-108-3-2012 IT-20-108-4-2012 IT-20-108-6-2012 IT-20-108-7-2012 IT-20-108-9-2012 IT-20-108-10-2012 IT-20-108-40-2013 IT-20-108-41-2013 IT-20-108-42-2013 IT-20-108-43-2013 IT-20-108-44-2013 IT-20-108-68-2012 IT-20-108-69-2012 IT-20-108-72-2013 IT-20-108-73-2013 IT-20-108-76-2013 IT-20-108-200-2013 IT-20-108-202-2013 IT-20-108-203-2013 IT-20-118-34-2012 IT-20-118-35-2012 IT-20-118-36-2012 IT-20-118-37-2012 IT-20-118-39-2012 IT-20-118-40-2012 IT-20-118-41-2012 IT-20-118-42-2012 IT-20-118-43-2012 IT-20-163-58-2012 IT-20-163-59-2012 IT-20-163-60-2012 IT-20-163-61-2012 IT-20-163-62-2012 IT-20-163-64-2012 IT-20-163-65-2012 IT-20-163-66-2012 IT-20-171-2-2013 IT-20-171-3-2013 IT-20-171-4-2013 IT-20-171-5-2013 IT-20-171-7-2013