64 daughter colonies of the 4a were tested:
DE-4-5-2139-2009 DE-4-5-2145-2009 DE-4-5-2146-2009 DE-4-5-2147-2009 DE-4-5-2148-2009 DE-4-5-2149-2009 DE-4-5-2150-2009 DE-4-5-2151-2009 DE-4-5-2153-2009 DE-4-5-2155-2009 DE-4-5-2156-2009 DE-4-5-2158-2009 DE-4-5-2170-2009 DE-4-5-2206-2010 DE-4-5-2207-2010 DE-4-5-2208-2010 DE-4-5-2209-2010 DE-4-5-2211-2010 DE-4-5-2212-2010 DE-4-5-2214-2010 DE-4-5-2215-2010 DE-4-5-2216-2010 DE-4-5-2217-2010 DE-4-5-2218-2010 DE-4-5-2219-2010 DE-4-5-2220-2010 DE-4-5-2222-2010 DE-4-5-2223-2010 DE-4-5-2224-2010 DE-4-5-2225-2010 DE-4-5-2226-2010 DE-4-5-2227-2010 DE-4-5-2228-2010 DE-4-5-2229-2010 DE-4-5-2230-2010 DE-4-5-2231-2010 DE-4-5-2234-2010 DE-4-5-2369-2011 DE-4-5-2370-2011 DE-4-5-2372-2011 DE-4-5-2373-2011 DE-4-5-2374-2011 DE-4-5-2376-2011 DE-4-5-2377-2011 DE-4-5-2381-2011 DE-4-5-2382-2011 DE-4-5-2383-2011 DE-4-5-2384-2011 DE-4-5-2385-2011 DE-4-5-2386-2011 DE-4-5-2387-2011 DE-4-5-2388-2011 DE-4-5-2476-2013 DE-4-5-2477-2013 DE-4-5-2478-2013 DE-4-5-2479-2013 DE-4-5-2480-2013 DE-4-5-2481-2013 DE-4-5-2482-2013 DE-4-5-2483-2013 DE-4-5-2484-2013 DE-4-5-2485-2013 DE-4-5-2486-2013 DE-4-5-2487-2013
15 tested queens were mated at the mating station:
DE-4-5-2410-2012 DE-4-5-2411-2012 DE-4-5-2415-2012 DE-4-5-2416-2012 DE-4-5-2417-2012 DE-4-5-2418-2012 DE-4-5-2450-2012 DE-4-5-2451-2012 DE-4-5-2452-2012 DE-4-5-2453-2012 DE-4-5-2454-2012 DE-4-5-2455-2012 DE-4-5-2456-2012 DE-4-5-2457-2012 DE-4-5-2458-2012
36 daughter colonies of queens mated at the mating station were tested:
DE-4-5-2519-2013 DE-4-5-2520-2013 DE-4-5-2521-2013 DE-4-5-2522-2013 DE-4-5-2523-2013 DE-4-5-2524-2013 DE-4-5-2525-2013 DE-4-5-2526-2013 DE-4-5-2576-2014 DE-4-5-2577-2014 DE-4-5-2578-2014 DE-4-5-2579-2014 DE-4-5-2580-2014 DE-4-5-2581-2014 DE-4-5-2582-2014 DE-4-5-2583-2014 DE-4-5-2588-2014 DE-4-5-2589-2014 DE-4-5-2590-2014 DE-4-5-2591-2014 DE-4-5-2592-2014 DE-4-5-2593-2014 DE-4-5-2594-2014 DE-4-5-2595-2014 DE-4-5-2596-2014 DE-4-5-2597-2014 DE-4-5-2598-2014 DE-4-5-2599-2014 DE-4-5-2670-2015 DE-4-5-2689-2015 DE-4-5-2690-2015 DE-4-5-2692-2015 DE-4-5-2693-2015 DE-4-5-2694-2015 DE-4-5-2697-2015 DE-4-5-2699-2015