7 daughter colonies of the 4a were tested:
DE-2-176-36-2010 DE-2-176-37-2010 DE-2-176-38-2010 DE-4-290-19-2010 DE-4-290-23-2010 DE-4-290-27-2010 DE-4-290-28-2010
21 tested queens were mated at the mating station:
IT-20-1-314-2011 IT-20-72-24-2011 IT-20-84-1-2011 IT-20-84-2-2011 IT-20-84-4-2011 IT-20-108-26-2011 IT-20-108-29-2011 IT-20-108-30-2011 IT-20-108-42-2011 IT-20-108-44-2011 IT-20-118-33-2011 IT-20-118-34-2011 IT-20-118-36-2011 IT-20-118-37-2011 IT-20-118-39-2011 IT-20-118-41-2011 IT-20-163-6-2011 IT-20-163-7-2011 IT-20-163-9-2011 IT-20-163-10-2011 IT-20-163-12-2011
15 daughter colonies of queens mated at the mating station were tested:
IT-20-72-38-2014 IT-20-72-40-2014 IT-20-72-41-2014 IT-20-72-42-2014 IT-20-72-43-2014 IT-20-72-44-2014 IT-20-72-45-2014 IT-20-72-46-2014 IT-20-72-47-2014 IT-20-163-22-2013 IT-20-163-24-2013 IT-20-163-26-2013 IT-20-163-28-2013 IT-20-163-29-2013 IT-20-163-16310-2013