9 daughter colonies of the 4a were tested:
DE-15-163-6-2011 DE-15-163-7-2011 DE-15-163-9-2011 DE-15-163-10-2011 DE-15-163-11-2011 DE-15-163-12-2011 DE-15-163-13-2011 DE-15-163-20-2012 DE-15-163-23-2012
20 tested queens were mated at the mating station:
DE-15-163-10-2013 DE-15-163-11-2013 DE-15-163-12-2013 DE-15-163-14-2013 DE-15-163-15-2013 DE-15-163-16-2013 DE-15-164-1-2013 DE-15-164-2-2013 DE-15-164-3-2013 DE-15-164-4-2013 DE-15-164-5-2013 DE-15-172-2-2013 DE-15-172-3-2013 DE-15-172-4-2013 DE-15-172-5-2013 DE-15-172-6-2013 DE-15-172-7-2013 DE-15-172-8-2013 DE-15-172-40-2013 DE-15-172-41-2013
2 daughter colonies of queens mated at the mating station were tested:
DE-15-172-55-2015 DE-15-172-66-2016